Crystallization and preliminary X-ray investigation of a complex between a Fab fragment and its antigen, cyclosporin

D Altschuh; H P Kocher; V F Quesniaux; D Schmitter; M H Van Regenmortel; J C Thierry

doi:10.1016/0022-2836(89)90181-2

Crystallization and preliminary X-ray investigation of a complex between a Fab fragment and its antigen, cyclosporin

J Mol Biol. 1989 Sep 5;209(1):177-8. doi: 10.1016/0022-2836(89)90181-2.

Authors

D Altschuh¹, H P Kocher, V F Quesniaux, D Schmitter, M H Van Regenmortel, J C Thierry

Affiliation

¹ Laboratoire d'Immunochimie, Institut de Biologie Moléculaire et Cellulaire du CNRS, Strasbourg, France.

PMID: 2810368
DOI: 10.1016/0022-2836(89)90181-2

Abstract

Preliminary crystallographic data are given for a complex between the cyclic undecapeptide cyclosporin and the Fab fragment of an anti-cyclosporin monoclonal antibody. Crystals of the complex are orthorhombic with space group P2(1)2(1)2(1) and diffract to 2.7 A resolution. The unit cell dimensions are a = 52.6 A, b = 70.2 A and c = 118.4 A. A native data set to 2.7 A resolution has been collected.

MeSH terms

Antibodies, Monoclonal
Crystallization
Cyclosporins*
Immunoglobulin Fab Fragments*
Macromolecular Substances
X-Ray Diffraction

Substances

Antibodies, Monoclonal
Cyclosporins
Immunoglobulin Fab Fragments
Macromolecular Substances