Objective: To study the effect of methamphetamine (METH) exposure on S-nitrosylation of protein disulphide isomerase and the neurotoxicity of METH in PC12 cells.
Methods: PC12 cells were exposed to different concentrations of METH, and the cell viability was assessed using the cell-counting kit-8. PC12 cells exposed to METH in the presence of the NOS inhibitor N-nitro-L-arginine (L-NNA) were examined for cell viability and S-nitrosylation of protein disulphide isomerase using the biotin-switch method, and the changes in cell morphology were examined with HE staining.
Results: METH exposure obviously decreased the cell viability and increased S-nitrosylation of protein disulphide isomerase, and the effect of METH was obviously inhibited by L-NNA treatment.
Conclusion: METH can cause obvious neurotoxicity and promote S-nitrosylation of protein disulphide isomerase in PC12 cells.
目的: 研究甲基苯丙胺（METH）对PC12细胞中二硫键异构酶（PDI）S亚硝基化的影响以及对神经元的毒性作用。
方法: 不同浓度的METH处理PC12细胞，CCK-8法检测细胞存活率，L-NNA与不同浓度的METH共同处理PC12细胞，生物素转化法检测各细胞内PDI及S亚硝基化PDI（PDI-SNO）表达情况，CCK-8法检测细胞存活率情况，HE染色法观察细胞形态学变化。结果 METH使得PC12细胞存活率降低，PDI-SNO表达率增高，当同时用一氧化氮合酶抑制剂N-硝基-L-精氨酸（L-NNA）与METH共同处理细胞时，L-NNA可有效地提高细胞的存活率，抑制PDI-SNO的表达率。
结论: METH可对PC12细胞产生明显毒性作用，且使PC12细胞中的PDI发生显著的S亚硝基化。