MALDI IMS and Cancer Tissue Microarrays

R Casadonte; R Longuespée; J Kriegsmann; M Kriegsmann

doi:10.1016/bs.acr.2016.11.007

MALDI IMS and Cancer Tissue Microarrays

Adv Cancer Res. 2017:134:173-200. doi: 10.1016/bs.acr.2016.11.007. Epub 2017 Jan 12.

Authors

R Casadonte¹, R Longuespée¹, J Kriegsmann², M Kriegsmann³

Affiliations

¹ Proteopath GmbH, Trier, Germany.
² Proteopath GmbH, Trier, Germany; Institute of Molecular Pathology, Trier, Germany; Center for Histology, Cytology and Molecular Diagnostics, Trier, Germany.
³ Institute of Pathology, University of Heidelberg, Heidelberg, Germany. Electronic address: [email protected].

PMID: 28110650
DOI: 10.1016/bs.acr.2016.11.007

Abstract

Matrix-assisted laser desorption/ionization imaging mass spectrometry (MALDI IMS) technology creates a link between the molecular assessment of numerous molecules and the morphological information about their special distribution. The application of MALDI IMS on formalin-fixed paraffin-embedded (FFPE) tissue microarrays (TMAs) is suitable for large-scale discovery analyses. Data acquired from FFPE TMA cancer samples in current research are very promising, and applications for routine diagnostics are under development. With the current rapid advances in both technology and applications, MALDI IMS technology is expected to enter into routine diagnostics soon. This chapter is intended to be comprehensive with respect to all aspects and considerations for the application of MALDI IMS on FFPE cancer TMAs with in-depth notes on technical aspects.

Keywords: Cancer; FFPE; IMS; MALDI; TMA; Tissue microarrays.

Publication types

Review

MeSH terms

Animals
Biomarkers, Tumor / metabolism*
Humans
Image Processing, Computer-Assisted / methods*
Molecular Imaging / methods*
Neoplasms / metabolism
Neoplasms / pathology*
Paraffin Embedding / methods
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods*
Tissue Array Analysis / methods*
Tissue Fixation / methods

Substances

Biomarkers, Tumor