The impact of a novel peach gum-derived polysaccharide on postprandial blood glucose control in streptozotocin-induced diabetic mice

Int J Biol Macromol. 2017 May:98:379-386. doi: 10.1016/j.ijbiomac.2017.01.085. Epub 2017 Jan 20.

Abstract

Peach [Prunus persica (L.)] gum exudates are produced by the trunks and fruits in peach gummosis. Clinically, these exudates have been used to treat diabetes in China, though the molecular mechanism underlying remains unclear. In the current study, a novel peach gum-derived polysaccharide was isolated, designated as PGPSD, and its anti-diabetic effect was assessed in mice. This polysaccharide was composed of arabinose, xylose and galactose in the molar ratio of 5.98:1:3.55, with the average molecular weight at 1.00×106Da. The animal study demonstrated that the PGPSD polysaccharide significantly lowered the postprandial blood glucose in streptozotocin-induced diabetic mice. Histology and immunohistochemistry results further confirmed that the application of PGPSD polysaccharide partially restored the pancreatic islets in diabetic mice, and enhanced the expression of pancreatic duodenal homeobox-1, insulin and hexokinase1. Collectively, the data suggested that the peach gum-derived polysaccharide had a meaningful potential as a non-insulin therapeutic compound in the treatment of diabetes.

Keywords: Blood glucose; Diabetes; Insulin; Pancreatic islet; Peach gum; Polysaccharide.

MeSH terms

  • 3T3-L1 Cells
  • Animals
  • Blood Glucose / metabolism*
  • Diabetes Mellitus, Experimental / blood*
  • Diabetes Mellitus, Experimental / drug therapy*
  • Diabetes Mellitus, Experimental / pathology
  • Hep G2 Cells
  • Humans
  • Hypoglycemic Agents / chemistry
  • Hypoglycemic Agents / pharmacology
  • Hypoglycemic Agents / therapeutic use
  • Hypoglycemic Agents / toxicity
  • Insulin / blood
  • Kidney / drug effects
  • Kidney / metabolism
  • Kidney / pathology
  • Male
  • Mice
  • Mice, Inbred C57BL
  • Molecular Weight
  • Monosaccharides / analysis
  • Pancreas / drug effects
  • Pancreas / metabolism
  • Pancreas / pathology
  • Plant Gums / chemistry*
  • Polysaccharides / chemistry
  • Polysaccharides / pharmacology*
  • Polysaccharides / therapeutic use
  • Polysaccharides / toxicity
  • Postprandial Period / drug effects*
  • Prunus persica / chemistry*

Substances

  • Blood Glucose
  • Hypoglycemic Agents
  • Insulin
  • Monosaccharides
  • Plant Gums
  • Polysaccharides