A SUMO Ligase AtMMS21 Regulates the Stability of the Chromatin Remodeler BRAHMA in Root Development

Juanjuan Zhang; Jianbin Lai; Feige Wang; Songguang Yang; Zhipeng He; Jieming Jiang; Qingliang Li; Qian Wu; Yiyang Liu; Mengyuan Yu; Jinju Du; Qi Xie; Keqiang Wu; Chengwei Yang

doi:10.1104/pp.17.00014

A SUMO Ligase AtMMS21 Regulates the Stability of the Chromatin Remodeler BRAHMA in Root Development

Plant Physiol. 2017 Mar;173(3):1574-1582. doi: 10.1104/pp.17.00014. Epub 2017 Jan 23.

Authors

Juanjuan Zhang^{1

2

3

4

5}, Jianbin Lai^{1

2

3

4

5}, Feige Wang^{1

2

3

4

5}, Songguang Yang^{1

2

3

4

5}, Zhipeng He^{1

2

3

4

5}, Jieming Jiang^{1

2

3

4

5}, Qingliang Li^{1

2

3

4

5}, Qian Wu^{1

2

3

4

5}, Yiyang Liu^{1

2

3

4

5}, Mengyuan Yu^{1

2

3

4

5}, Jinju Du^{1

2

3

4

5}, Qi Xie^{1

2

3

4

5}, Keqiang Wu^{1

2

3

4

5}, Chengwei Yang^{6

7

8

9

10}

Affiliations

¹ Guangdong Provincial Key Laboratory of Biotechnology for Plant Development, School of Life Science, South China Normal University, Guangzhou 510631, China (J.Z., J.L., F.W., Z.H., J.J., Q.W., Y.L., M.Y., J.D., C.Y.).
² Key Laboratory of South China Agricultural Plant Molecular Analysis and Gene Improvement, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou 510650, China (S.Y.).
³ State Key Laboratory of Plant Genomics, National Centre for Plant Gene Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China (Q.L., Q.X.).
⁴ Graduate University of Chinese Academy of Sciences, Beijing 100049, China (Q.L., Q.X.); and.
⁵ Institute of Plant Biology, National Taiwan University, Taipei 106, Taiwan (K.W.).
⁶ Guangdong Provincial Key Laboratory of Biotechnology for Plant Development, School of Life Science, South China Normal University, Guangzhou 510631, China (J.Z., J.L., F.W., Z.H., J.J., Q.W., Y.L., M.Y., J.D., C.Y.); [email protected].
⁷ Key Laboratory of South China Agricultural Plant Molecular Analysis and Gene Improvement, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou 510650, China (S.Y.); [email protected].
⁸ State Key Laboratory of Plant Genomics, National Centre for Plant Gene Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China (Q.L., Q.X.); [email protected].
⁹ Graduate University of Chinese Academy of Sciences, Beijing 100049, China (Q.L., Q.X.); and [email protected].
¹⁰ Institute of Plant Biology, National Taiwan University, Taipei 106, Taiwan (K.W.) [email protected].

Abstract

Chromatin remodeling is essential for gene expression regulation in plant development and response to stresses. Brahma (BRM) is a conserved ATPase in the SWI/SNF chromatin remodeling complex and is involved in various biological processes in plant cells, but the regulation mechanism on BRM protein remains unclear. Here, we report that BRM interacts with AtMMS21, a SUMO ligase in Arabidopsis (Arabidopsis thaliana). The interaction was confirmed in different approaches in vivo and in vitro. The mutants of BRM and AtMMS21 displayed a similar defect in root development. In the mms21-1 mutant, the protein level of BRM-GFP was significantly lower than that in wild type, but the RNA level of BRM did not change. Biochemical evidence indicated that BRM was modified by SUMO3, and the reaction was enhanced by AtMMS21. Furthermore, overexpression of wild-type AtMMS21 but not the mutated AtMMS21 without SUMO ligase activity was able to recover the stability of BRM in mms21-1 Overexpression of BRM in mms21-1 partially rescued the developmental defect of roots. Taken together, these results supported that AtMMS21 regulates the protein stability of BRM in root development.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Adenosine Triphosphatases / genetics*
Adenosine Triphosphatases / metabolism
Arabidopsis / genetics
Arabidopsis / growth & development
Arabidopsis / metabolism
Arabidopsis Proteins / genetics*
Arabidopsis Proteins / metabolism
Chromatin Assembly and Disassembly / genetics
Gene Expression Regulation, Developmental*
Gene Expression Regulation, Plant*
Immunoblotting
Ligases / genetics*
Ligases / metabolism
Microscopy, Confocal
Models, Genetic
Mutation
Plant Roots / genetics*
Plant Roots / growth & development
Plant Roots / metabolism
Plants, Genetically Modified
Protein Binding
Protein Stability
Reverse Transcriptase Polymerase Chain Reaction

Substances

Arabidopsis Proteins
Adenosine Triphosphatases
BRM protein, Arabidopsis
Ligases
MMS21 protein, Arabidopsis