Comparison of two rapid biochemical tests and four chromogenic selective media for detection of carbapenemase-producing Gram-negative bacteria

J Microbiol Methods. 2017 Apr:135:66-68. doi: 10.1016/j.mimet.2017.01.012. Epub 2017 Jan 25.

Abstract

We evaluated RAPIDEC® CARBA NP, Neo-Rapid CARB, chromID® CARBA SMART (CARB/OXA), Brilliance™ CRE/ESBL, ChromArt CRE and BBL™ CHROMagar™ CPE for the detection of carbapenemase-producing bacteria. The analytical sensitivity of RAPIDEC® CARBA NP was better than that of Neo-Rapid CARB. A combination of carbapenemase and ESBL screening plates could be advantageous.

Keywords: Biochemical screening; Carbapenemase; Carbapenemase-producing Gram-negative bacteria; Chromogenic selective media; Resistance.

MeSH terms

  • Bacterial Proteins / analysis*
  • Bacteriological Techniques / instrumentation*
  • Bacteriological Techniques / methods*
  • Chromogenic Compounds
  • Colorimetry / methods
  • Culture Media / chemistry
  • Gram-Negative Bacteria / classification
  • Gram-Negative Bacteria / enzymology*
  • Gram-Negative Bacteria / growth & development
  • Gram-Negative Bacterial Infections / diagnosis*
  • Gram-Negative Bacterial Infections / microbiology
  • Humans
  • Reagent Kits, Diagnostic
  • Sensitivity and Specificity
  • Time Factors
  • beta-Lactamases / analysis*

Substances

  • Bacterial Proteins
  • Chromogenic Compounds
  • Culture Media
  • Reagent Kits, Diagnostic
  • beta-Lactamases
  • carbapenemase