Objective: To investigate the expression of long non-coding RNA (lncRNA) SNHG8 in EB virus related gastric cancer and their correlation prognosis. Methods: The expression of SNHG8 in 93 gastric cancers and 93 cancer-free controls, matched by age and sex, were determined by real-time PCR. EB virus expression was detected by EBER in situ hybridization. Results: Forty-one gastric cancers were EB virus associated. For all gastric cancers, SNHG8 expression was 14 times higher (P=0.001) than that in non-cancer controls; in the EB virus related gastric cancers, SNHG8 expression was increased 25 times (P<0.05) over EB virus negative gastric cancers. SNHG8 expression level was also significantly associated with TNM staging (P<0.05). Conclusions: SNHG8 may act as a proto-oncogene, participating in gastric carcinogenesis.EB virus infection of gastric mucosa may promote SNHG8 expression.
目的： 探讨长链非编码RNA SNHG8在EB病毒相关胃癌中的表达及其临床意义。 方法： 收集2012年6月至2015年4月于福建省肿瘤医院进行外科手术的93例胃癌病例(癌组织和癌旁组织)；采用EB病毒编码的小RNA(EBER)原位杂交检测EB病毒的表达；即时荧光定量PCR验证上述组织中SNHG8的表达，并分析其与临床病理特征的关系。 结果： 胃癌细胞核阳性的病例定义为EB病毒相关胃癌，共41例；93例胃癌患者中，SNHG8在肿瘤组织的表达比癌旁组织上调14倍(P＝0.001)；同时EB病毒相关胃癌组织SNHG8表达比EB病毒阴性胃癌组织表达上调25倍(P＝0.027)。EB病毒相关胃癌肿瘤组织中SNHG8的表达水平与TNM分期显著相关(P<0.05)。 结论： SNHG8可能作为一个原癌基因，参与胃癌的发生发展；EB病毒感染胃黏膜可能促进SNHG8的表达，为EB病毒相关胃癌的致病机制研究提供新思路。.
Keywords: Herpesvirus 4, human; Long-non-coding RNA; Stomach neoplasms.