A rapid and robust UHPLC-DAD method for the quantification of amphotericin B in human plasma

J Pharm Biomed Anal. 2017 May 10:138:142-145. doi: 10.1016/j.jpba.2017.01.048. Epub 2017 Feb 8.

Abstract

Amphotericin B is an antifungal drug widely used in Intensive Care Units. Therapeutic drug monitoring (TDM) of amphotericin B is recommended for the assessment of toxicity surveillance and treatment optimization. In this paper we described the development and validation of a new Ultra High Performance Liquid Chromatography coupled to Diode Array Detection (UHPLC-DAD) method for the quantification of Amphotericin B in 200μL human plasma over a wide range of concentrations (0.125-10mg/L). The new method has been validated following international guidelines on bioanalytical method validation and showed high selectivity, high accuracy and precision and high process efficiency. The new UHPLC-DAD method that we describe is robust, rapid, cost effective and suitable for application to the routine TDM analyses.

Keywords: Amphotericin B; UHPLC.

MeSH terms

  • Adolescent
  • Amphotericin B / blood*
  • Amphotericin B / chemistry*
  • Antifungal Agents / blood
  • Antifungal Agents / chemistry
  • Child
  • Child, Preschool
  • Chromatography, High Pressure Liquid / methods*
  • Drug Monitoring / methods
  • Female
  • Humans
  • Infant
  • Male
  • Plasma / chemistry*
  • Reproducibility of Results

Substances

  • Antifungal Agents
  • Amphotericin B