Streptococcus pneumoniae polA gene is expressed in Escherichia coli and can functionally substitute for the E. coli polA gene

P López; S Martinez; A Diaz; M Espinosa

doi:10.1128/jb.169.10.4869-4871.1987

Streptococcus pneumoniae polA gene is expressed in Escherichia coli and can functionally substitute for the E. coli polA gene

J Bacteriol. 1987 Oct;169(10):4869-71. doi: 10.1128/jb.169.10.4869-4871.1987.

Authors

P López¹, S Martinez, A Diaz, M Espinosa

Affiliation

¹ Centro de Investigaciones Biológicas, Consejo Superior de Investigaciones Científicas, Madrid, Spain.

Abstract

The Streptococcus pneumoniae polA+ gene was introduced into Escherichia coli on the recombinant plasmid pSM31, which is based on the pSC101 replicon. Extracts of E. coli polA5 mutants containing pSM31 showed DNA polymerase activity, indicating that the pneumococcal DNA polymerase I was expressed in the heterospecific host. Complete complementation of the E. coli polA5 mutation by the pneumococcal polA+ gene was detected in excision repair of DNA damage.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Cloning, Molecular
DNA Polymerase I / genetics*
DNA Polymerase I / metabolism
DNA Repair
DNA Restriction Enzymes
DNA, Recombinant
Electrophoresis, Polyacrylamide Gel
Escherichia coli / enzymology
Escherichia coli / genetics*
Gene Expression Regulation
Genes, Bacterial*
Mutation
Plasmids
Streptococcus pneumoniae / enzymology
Streptococcus pneumoniae / genetics*
Transcription, Genetic
Transformation, Bacterial

Substances

DNA, Recombinant
DNA Polymerase I
DNA Restriction Enzymes