Enzyme-Controlled Nanodevice for Acetylcholine-Triggered Cargo Delivery Based on Janus Au-Mesoporous Silica Nanoparticles

Chemistry. 2017 Mar 28;23(18):4276-4281. doi: 10.1002/chem.201700603. Epub 2017 Mar 15.

Abstract

This work reports a new gated nanodevice for acetylcholine-triggered cargo delivery. We prepared and characterized Janus Au-mesoporous silica nanoparticles functionalized with acetylcholinesterase on the Au face and with supramolecular β-cyclodextrin:benzimidazole inclusion complexes as caps on the mesoporous silica face. The nanodevice is able to selectively deliver the cargo in the presence of acetylcholine via enzyme-mediated acetylcholine hydrolysis, locally lowering the pH and opening the supramolecular gate. Given the key role played by ACh and its relation with Parkinson's disease and other nervous system diseases, we believe that these findings could help design new therapeutic strategies.

Keywords: acetylcholine; controlled release; mesoporous materials; nanoparticles; nanotechnology.

MeSH terms

  • Acetylcholine / metabolism
  • Acetylcholinesterase / chemistry
  • Acetylcholinesterase / metabolism*
  • Benzimidazoles / chemistry
  • Cell Survival / drug effects
  • Doxorubicin / chemistry
  • Doxorubicin / metabolism
  • Doxorubicin / toxicity
  • Drug Carriers / chemistry*
  • Enzymes, Immobilized / chemistry
  • Enzymes, Immobilized / metabolism
  • Gold / chemistry*
  • HeLa Cells
  • Humans
  • Hydrogen-Ion Concentration
  • Hydrolysis
  • Nanoparticles / chemistry*
  • Organometallic Compounds / chemistry
  • Organometallic Compounds / metabolism
  • Porosity
  • Silicon Dioxide / chemistry*
  • beta-Cyclodextrins / chemistry

Substances

  • Benzimidazoles
  • Drug Carriers
  • Enzymes, Immobilized
  • MCM-41
  • Organometallic Compounds
  • beta-Cyclodextrins
  • tris(2,2'-bipyridyl)ruthenium(II)
  • Gold
  • Silicon Dioxide
  • Doxorubicin
  • benzimidazole
  • Acetylcholinesterase
  • betadex
  • Acetylcholine