Phenylhydrazine stimulates lymphopoiesis and accelerates Abelson murine leukemia virus-induced pre-B cell lymphomas

J Immunol. 1987 Nov 1;139(9):3091-8.

Abstract

Infection of bone marrow or fetal liver cells with Abelson murine leukemia virus (A-MuLV) results in the transformation of pre-B cells and the development of erythroid colonies, indicating that the abl oncogene can affect the growth characteristics of immature cells in both the B cell and erythroid lineages. By comparison, infection of mice with A-MuLV results primarily in the development of pre-B cell lymphomas. To determine whether A-MuLV could induce erythroid disease in vivo, NFS/N mice were pretreated with phenylhydrazine (PHZ) to stimulate erythropoiesis and increase the frequency of potential target cells for A-MuLV. No erythroleukemias developed in mice treated with PHZ. Instead, the latency for pre-B cell lymphomas was reduced by half. This acceleration of disease could be attributed to a marked increase in pre-B cells as targets for transformation by A-MuLV in the bone marrows but not the spleens of treated mice. Increases in the frequencies of T cells in bone marrow and spleen also followed treatment with PHZ. These results show that although PHZ-induced anemia stimulates the production of T and B cells as well as erythroid progenitors, PHZ-treated mice do not develop erythroleukemia or T cell lymphomas. It was also found that the genetically determined resistance of adult C57BL/6 mice to lymphoma induction by A-MuLV could not be overcome by pretreatment with PHZ even though the frequency of pre-B cells in bone marrow was greatly increased by this treatment.

MeSH terms

  • Abelson murine leukemia virus*
  • Animals
  • Antigens, Differentiation, B-Lymphocyte / analysis
  • Antigens, Differentiation, T-Lymphocyte / analysis
  • B-Lymphocytes* / cytology
  • B-Lymphocytes* / immunology
  • Cell Differentiation
  • Clone Cells
  • Hematopoiesis / drug effects*
  • Leukemia Virus, Murine*
  • Lymphoma / etiology*
  • Lymphoma / pathology
  • Mice
  • Mice, Inbred Strains
  • Neoplasms, Experimental / etiology
  • Neoplasms, Experimental / pathology
  • Oncogenes
  • Phenylhydrazines / pharmacology*
  • Time Factors

Substances

  • Antigens, Differentiation, B-Lymphocyte
  • Antigens, Differentiation, T-Lymphocyte
  • Phenylhydrazines
  • phenylhydrazine