A cDNA clone expressing a rhoptry protein of Plasmodium falciparum

R L Coppel; A E Bianco; J G Culvenor; P E Crewther; G V Brown; R F Anders; D J Kemp

doi:10.1016/0166-6851(87)90020-x

A cDNA clone expressing a rhoptry protein of Plasmodium falciparum

Mol Biochem Parasitol. 1987 Aug;25(1):73-81. doi: 10.1016/0166-6851(87)90020-x.

Authors

R L Coppel¹, A E Bianco, J G Culvenor, P E Crewther, G V Brown, R F Anders, D J Kemp

Affiliation

¹ Walter and Eliza Hall Institute of Medical Research, Victoria, Australia.

PMID: 2823136
DOI: 10.1016/0166-6851(87)90020-x

Abstract

Antibodies from immune humans were used to select a cDNA clone expressing an asexual blood stage antigen of Plasmodium falciparum. The expressed fused polypeptide was used as an affinity reagent to purify human antibodies specific for the corresponding parasite antigen. Western blotting and immunoelectronmicroscopy demonstrated that the antigen was a 105 kDa protein located in the rhoptries of merozoites. The cDNA encodes the carboxy terminus of the rhoptry antigen, a sequence rich both in charged and hydroxy amino acids.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Amino Acid Sequence
Animals
Antigens, Protozoan / genetics*
Base Sequence
Chromatography, Affinity
Cloning, Molecular
DNA / genetics*
DNA Restriction Enzymes
Deoxyribonuclease EcoRI
Deoxyribonuclease HindIII
Fluorescent Antibody Technique
Genes
Humans
Immunoassay
Microscopy, Electron
Molecular Sequence Data
Nucleic Acid Hybridization
Plasmodium falciparum / genetics*
Plasmodium falciparum / immunology
Plasmodium falciparum / ultrastructure

Substances

Antigens, Protozoan
DNA
DNA Restriction Enzymes
Deoxyribonuclease EcoRI
Deoxyribonuclease HindIII

Associated data

GENBANK/M17530