The Salmonella effector SseJ disrupts microtubule dynamics when ectopically expressed in normal rat kidney cells

PLoS One. 2017 Feb 24;12(2):e0172588. doi: 10.1371/journal.pone.0172588. eCollection 2017.

Abstract

Salmonella effector protein SseJ is secreted by Salmonella into the host cell cytoplasm where it can then modify host cell processes. Whilst host cell small GTPase RhoA has previously been shown to activate the acyl-transferase activity of SseJ we show here an un-described effect of SseJ protein production upon microtubule dynamism. SseJ prevents microtubule collapse and this is independent of SseJ's acyl-transferase activity. We speculate that the effects of SseJ on microtubules would be mediated via its known interactions with the small GTPases of the Rho family.

MeSH terms

  • Acyltransferases / genetics*
  • Acyltransferases / metabolism
  • Amino Acid Sequence
  • Animals
  • Bacterial Proteins / genetics*
  • Bacterial Proteins / metabolism
  • Binding Sites
  • Cell Line
  • Epithelial Cells / microbiology*
  • Epithelial Cells / ultrastructure
  • Gene Expression Regulation
  • Genomic Islands
  • Genomic Library
  • Host-Pathogen Interactions*
  • Humans
  • Immunoprecipitation
  • Kidney / microbiology
  • Kidney / pathology
  • Macrophages / microbiology
  • Macrophages / ultrastructure
  • Microtubules / microbiology*
  • Microtubules / ultrastructure
  • Protein Binding
  • Rats
  • Saccharomyces cerevisiae / genetics
  • Saccharomyces cerevisiae / metabolism
  • Salmonella typhimurium / genetics*
  • Salmonella typhimurium / metabolism
  • Salmonella typhimurium / pathogenicity
  • Sequence Alignment
  • Signal Transduction
  • rho GTP-Binding Proteins / genetics*
  • rho GTP-Binding Proteins / metabolism

Substances

  • Bacterial Proteins
  • Acyltransferases
  • RhoA protein, rat
  • rho GTP-Binding Proteins