Abstract
Mesenchymal stromal cells from deciduous teeth in 2D- and 3D-cultures on culture plastic, silicate glass, porous polystyrene, and experimental polylactoglycolide matrices were visualized by scanning electron microscopy with lanthanide contrasting. Supravital staining of cell cultures with a lanthanide-based dye (neodymium chloride) preserved normal cell morphology and allowed assessment of the matrix properties of the carriers. The developed approach can be used for the development of biomaterials for tissue engineering.
Keywords:
lanthanide; mesenchymal stromal cells; scanning electron microscopy; tissue engineering.
MeSH terms
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Biocompatible Materials / chemistry
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Biocompatible Materials / pharmacology
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Cell Culture Techniques
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Cell Differentiation / drug effects
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Cell Proliferation / drug effects
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Contrast Media / chemistry*
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Dental Pulp / cytology
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Dental Pulp / drug effects
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Glass / chemistry
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Humans
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Mesenchymal Stem Cells / drug effects
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Mesenchymal Stem Cells / ultrastructure*
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Microscopy, Electron, Scanning / methods*
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Neodymium / chemistry*
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Osteogenesis
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Plastics / chemistry
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Plastics / pharmacology
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Polyesters / chemistry
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Polyesters / pharmacology
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Polystyrenes / chemistry
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Polystyrenes / pharmacology
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Primary Cell Culture
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Staining and Labeling / methods
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Tissue Engineering
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Tissue Scaffolds / chemistry*
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Tooth, Deciduous / cytology
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Tooth, Deciduous / drug effects
Substances
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Biocompatible Materials
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Contrast Media
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Plastics
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Polyesters
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Polystyrenes
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neodymium chloride
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Neodymium
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poly(lactide)