Cloning and characterization of the metallothionein-I gene from mouse LMTK cells

Cytobios. 1987;52(208):33-8.

Abstract

A clone of about 14 kb containing the metallothionein MT-I gene and three repetitive sequences, was isolated from a genomic library of mouse LMTK DNA. The MT-I gene was functional. Transfected cells became cadmium resistant. Two of the three repetitive sequences were moderately repetitive while the other was closely related to the R family.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cadmium / toxicity
  • Cadmium Chloride
  • Cell Line
  • Cell Survival / drug effects
  • Cloning, Molecular*
  • DNA Restriction Enzymes
  • Genes*
  • Metallothionein / genetics*
  • Mice
  • Nucleotide Mapping

Substances

  • Cadmium
  • Metallothionein
  • DNA Restriction Enzymes
  • Cadmium Chloride