Calmodulin-binding protein CBP60g functions as a negative regulator in Arabidopsis anthocyanin accumulation

PLoS One. 2017 Mar 2;12(3):e0173129. doi: 10.1371/journal.pone.0173129. eCollection 2017.

Abstract

Anthocyanins, a kind of flavonoid, normally accumulate in the flowers and fruits and make them colorful. Anthocyanin accumulation is regulated via the different temporal and spatial expression of anthocyanin regulatory and biosynthetic genes. CBP60g, a calmodulin binding protein, has previously been shown to have a role in pathogen resistance, drought tolerance and ABA sensitivity. In this study, we found that CBP60g repressed anthocyanin accumulation induced by drought, sucrose and kinetin. The expression pattern of CBP60g was in accordance with the anthocyanin accumulation tissues. Real-time qPCR analysis revealed that the anthocyanin biosynthetic genes CHS, CHI and DFR, as well as two members of MBW complex, PAP1, a MYB transcription factor, and TT8, a bHLH transcription factor, were down regulated by CBP60g.

MeSH terms

  • Anthocyanins / metabolism*
  • Arabidopsis / metabolism*
  • Arabidopsis / physiology
  • Calmodulin-Binding Proteins / metabolism*
  • Droughts
  • Pancreatitis-Associated Proteins
  • Stress, Physiological

Substances

  • Anthocyanins
  • Calmodulin-Binding Proteins
  • Pancreatitis-Associated Proteins
  • REG3A protein, human

Grants and funding

This work was supported by National Natural Science Foundation of China (No. 31360056), and Inner Mongolia Science & Technology Plan-Innovation Team (20150304) to Guojing Li. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.