Histopathological assessment of OASIS Ultra on critical-sized wound healing: a pilot study

J Cutan Pathol. 2017 Jun;44(6):523-529. doi: 10.1111/cup.12925. Epub 2017 Mar 23.

Abstract

Background: Dermatopathologists assess wounds secondary to trauma, infection, or oncologic resection that can be challenging to reconstruct. OASIS Ultra, an extracellular matrix, has been described for use in chronic and burn wounds. The aim of this pilot study is to assess wound healing in post-traumatic and infective wounds treated with OASIS using histological markers of repair.

Materials and methods: Adults with traumatic, infective or iatrogenic wound defects with size precluding primary closure were eligible. Half the wound was randomly assigned to receive OASIS plus standard therapy; the other half received standard of care (SOC) therapy. During dressing changes, standardized-scale photographs were taken and biopsies obtained. Histologic sections were reviewed for degree of acute inflammation and extent of tissue repair. Neutrophils, edema, hemorrhage, necrosis, fibroblasts, collagen density and neovascularization were semi-quantitatively assessed.

Results: Forty-four skin biopsies from 7 patients with 10 acute wounds met eligibility criteria. Histologically, OASIS samples demonstrated improved acute inflammation scores compared to SOC. No patients experienced OASIS-related complications. OASIS-treated wound halves trended toward more wound contraction and improved tissue repair.

Conclusion: Our scoring system aids histopathological wound assessment. Treatment of critical-sized, post-traumatic, acute wounds with OASIS resulted in decreased inflammation, and potentially more advanced wound healing, compared to SOC.

Keywords: OASIS; acute inflammation; extracellular matrix; fibrosis; wound healing.

Publication types

  • Randomized Controlled Trial

MeSH terms

  • Adult
  • Aged
  • Aged, 80 and over
  • Bandages
  • Biopsy
  • Burns* / metabolism
  • Burns* / pathology
  • Burns* / therapy
  • Chronic Disease
  • Extracellular Matrix* / metabolism
  • Extracellular Matrix* / pathology
  • Female
  • Humans
  • Male
  • Pilot Projects
  • Wound Healing*
  • Wounds and Injuries* / metabolism
  • Wounds and Injuries* / pathology
  • Wounds and Injuries* / therapy