Rapid typing of Mannheimia haemolytica major genotypes 1 and 2 using MALDI-TOF mass spectrometry

John Dustin Loy; Michael L Clawson

doi:10.1016/j.mimet.2017.03.002

Rapid typing of Mannheimia haemolytica major genotypes 1 and 2 using MALDI-TOF mass spectrometry

J Microbiol Methods. 2017 May:136:30-33. doi: 10.1016/j.mimet.2017.03.002. Epub 2017 Mar 4.

Authors

John Dustin Loy¹, Michael L Clawson²

Affiliations

¹ University of Nebraska-Lincoln, Institute of Agriculture and Natural Resources, School of Veterinary Medicine and Biomedical Sciences, Lincoln, NE, USA. Electronic address: [email protected].
² United States Department of Agriculture, Agricultural Research Service, U.S. Meat Animal Research Center, Clay Center, NE, USA. Electronic address: [email protected].

PMID: 28267571
DOI: 10.1016/j.mimet.2017.03.002

Abstract

Genotype 2M. haemolytica predominantly associate over genotype 1 with the lungs of cattle with respiratory disease and ICEs containing antimicrobial resistance genes. Distinct protein masses were detected by MALDI-TOF MS between genotype 1 and 2 strains. MALDI-TOF MS could rapidly differentiate genotype 2 strains in veterinary diagnostic laboratories.

Keywords: Bovine respiratory disease; Genotypes; MALDI-TOF mass spectrometry; Mannheimia haemolytica.

Publication types

Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

Animals
Bacterial Proteins / genetics
Cattle
Cattle Diseases / microbiology
DNA, Bacterial / genetics
Genes, MDR / genetics
Genotype*
Lung / microbiology
Mannheimia haemolytica / classification
Mannheimia haemolytica / genetics*
Mannheimia haemolytica / isolation & purification*
Mannheimia haemolytica / pathogenicity
Pasteurellaceae Infections / diagnosis
Pasteurellaceae Infections / microbiology
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods*

Substances

Bacterial Proteins
DNA, Bacterial

Grants and funding

P30 GM103509/GM/NIGMS NIH HHS/United States