Comparison of the artus Epstein-Barr virus (EBV) PCR kit and the Abbott RealTime EBV assay for measuring plasma EBV DNA loads in allogeneic stem cell transplant recipients

Víctor Vinuesa; Carlos Solano; Estela Giménez; David Navarro

doi:10.1016/j.diagmicrobio.2017.02.010

Comparison of the artus Epstein-Barr virus (EBV) PCR kit and the Abbott RealTime EBV assay for measuring plasma EBV DNA loads in allogeneic stem cell transplant recipients

Diagn Microbiol Infect Dis. 2017 May;88(1):36-38. doi: 10.1016/j.diagmicrobio.2017.02.010. Epub 2017 Feb 24.

Authors

Víctor Vinuesa¹, Carlos Solano², Estela Giménez¹, David Navarro³

Affiliations

¹ Microbiology Service, Hospital Clínico Universitario, Fundación INCLIVA, Valencia, Spain.
² Haematology Service, Hospital Clínico Universitario, Fundación INCLIVA, Valencia, Spain.
³ Microbiology Service, Hospital Clínico Universitario, Fundación INCLIVA, Valencia, Spain; Department of Microbiology, School of Medicine, University of Valencia, Spain. Electronic address: [email protected].

PMID: 28274502
DOI: 10.1016/j.diagmicrobio.2017.02.010

Abstract

The ability of the artus Epstein-Barr virus (EBV) PCR kit and the Abbott RealTime EBV PCR assay to detect and quantify plasma EBV DNAemia was compared. The agreement between these assays was 95.8%. The EBV DNA loads measured by the two assays significantly correlated (P=< 0.0001).

Keywords: Commercially-available EBV PCR assays; Epstein–Barr virus; Plasma EBV DNAemia; Real-time PCR.

Publication types

Comparative Study
Evaluation Study

MeSH terms

Adult
DNA, Viral / blood*
DNA, Viral / genetics
Epstein-Barr Virus Infections / virology*
Herpesvirus 4, Human / isolation & purification*
Humans
Molecular Diagnostic Techniques / methods*
Plasma / virology
Stem Cell Transplantation
Transplant Recipients
Transplantation, Homologous
Viral Load / methods*

Substances

DNA, Viral