5-Hydroxymethylcytosine (5hmC) and Ten-eleven translocation 1-3 (TET1-3) proteins in the dorsal root ganglia of mouse: Expression and dynamic regulation in neuropathic pain

Somatosens Mot Res. 2017 Jun;34(2):72-79. doi: 10.1080/08990220.2017.1292237. Epub 2017 Mar 1.

Abstract

Epigenetic mechanisms are increasingly implicated in chronic pain pathology. In this study, we demonstrate that the novel epigenetic mark 5-hydroxymethylcytosine (5hmC) is present in dorsal root ganglia (DRG) neurons and glia, and its levels increase following nerve injury. Furthermore, we show that the 5hmC-generating Ten-eleven translocation 1-3 (TET1-3) proteins are expressed in a cell-type specific manner in the DRG, with Tet3 displaying differential upregulation after injury, suggesting a potential role in neuropathic pain.

Keywords: Chronic pain; epigenetics; neuropathic pain.

MeSH terms

  • 5-Methylcytosine / analogs & derivatives*
  • 5-Methylcytosine / metabolism
  • Animals
  • DNA-Binding Proteins / metabolism*
  • Dioxygenases
  • Epigenesis, Genetic / physiology*
  • Ganglia, Spinal / metabolism*
  • Male
  • Mice
  • Mice, Inbred C57BL
  • Neuralgia / metabolism*
  • Proto-Oncogene Proteins / metabolism*

Substances

  • DNA-Binding Proteins
  • Proto-Oncogene Proteins
  • TET1 protein, mouse
  • 5-hydroxymethylcytosine
  • 5-Methylcytosine
  • Dioxygenases
  • Tet2 protein, mouse
  • Tet3 protein, mouse