Eight new γ-lactam alkaloids from the roots of the Hemerocallis minor Mill

Fitoterapia. 2017 Apr:118:80-86. doi: 10.1016/j.fitote.2017.03.001. Epub 2017 Mar 10.

Abstract

Eight new γ-lactam alkaloids, hemerominors A-H (1-8), including two pair of epimers (1-4), together with six known compounds (9-14) were isolated from the roots of Hemerocallis minor Mill. The structures of 1-8 were established on the basis of extensive NMR studies and HR-MS measurements as well as comparison with literature data. The absolute configurations of 1-8 were determined by CD spectral analysis and modified Mosher's method. All of compounds were evaluated for their inhibitory effects against LPS-induced NO production in RAW264.7 macrophages. Among them, compound 13 exhibited moderate inhibitory activity against NO production and with IC50 value of 18.0 μM.

Keywords: Anti-inflammatory activities; Hemerocallis minor Mill.; γ-lactam alkaloids.

MeSH terms

  • Alkaloids / chemistry*
  • Alkaloids / isolation & purification
  • Animals
  • Hemerocallis / chemistry*
  • Lactams / chemistry*
  • Lactams / isolation & purification
  • Macrophages / drug effects
  • Mice
  • Molecular Structure
  • Nitric Oxide
  • Plant Roots / chemistry
  • RAW 264.7 Cells

Substances

  • Alkaloids
  • Lactams
  • Nitric Oxide