Abstract
Bioassay-guided isolation of the secondary metabolites from the fungus Dichotomomyces sp. L-8 associated with the soft coral Lobophytum crassum led to the discovery of two new compounds, dichotones A and B (1 and 2), together with four known compounds including dichotocejpin C (3), bis-N-norgliovictin (4), bassiatin (5) and (3R,6R)-bassiatin (6). The structures of these compounds were determined by 1D, 2D NMR and mass spectrometry. (3R,6R)-bassiatin (6) displayed significant cytotoxic activities against the human breast cancer cell line MDA-MB-435 and the human lung cancer cell line Calu3 with IC50 values of 7.34 ± 0.20 and 14.54 ± 0.01 μM, respectively, while bassiatin (5), the diastereomer of compound 6, was not cytotoxic.
Keywords:
Dichotomomyces sp.; cytotoxic activity; secondary metabolites; structure elucidation.
MeSH terms
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Antineoplastic Agents, Phytogenic / chemistry*
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Antineoplastic Agents, Phytogenic / isolation & purification
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Antineoplastic Agents, Phytogenic / pharmacology
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Aquatic Organisms
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Cell Line, Tumor
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Cell Survival / drug effects
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Diketopiperazines / chemistry*
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Diketopiperazines / isolation & purification
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Diketopiperazines / pharmacology
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Humans
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Inhibitory Concentration 50
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Magnetic Resonance Spectroscopy
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Molecular Structure
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Morpholines / chemistry*
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Morpholines / isolation & purification
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Morpholines / pharmacology
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Saccharomycetales / chemistry
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Saccharomycetales / metabolism*
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Secondary Metabolism / physiology*
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Structure-Activity Relationship
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Sulfides / chemistry*
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Sulfides / isolation & purification
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Sulfides / pharmacology
Substances
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Antineoplastic Agents, Phytogenic
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Diketopiperazines
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Morpholines
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Sulfides
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bis-N-norgliovictin
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lateritin