Bapx1 mediates transforming growth factor-β- induced epithelial-mesenchymal transition and promotes a malignancy phenotype of gastric cancer cells

Shi Ouyang; Guodong Zhu; Lei Ouyang; Yuhao Luo; Rui Zhou; Changqie Pan; Jianping Bin; Yulin Liao; Wangjun Liao

doi:10.1016/j.bbrc.2017.03.029

Bapx1 mediates transforming growth factor-β- induced epithelial-mesenchymal transition and promotes a malignancy phenotype of gastric cancer cells

Biochem Biophys Res Commun. 2017 Apr 29;486(2):285-292. doi: 10.1016/j.bbrc.2017.03.029. Epub 2017 Mar 14.

Authors

Shi Ouyang¹, Guodong Zhu², Lei Ouyang³, Yuhao Luo⁴, Rui Zhou⁴, Changqie Pan⁴, Jianping Bin⁵, Yulin Liao⁵, Wangjun Liao⁶

Affiliations

¹ Department of Oncology, Nanfang Hospital, Southern Medical University Guangzhou, 510515, China; Key Laboratory of Liver Disease, Center of Infectious Diseases, PLA 458 Hospital, Guangzhou, 510602, China.
² Department of Oncology, Nanfang Hospital, Southern Medical University Guangzhou, 510515, China; Guangzhou First People's Hospital, Guangzhou Medical University, Guangzhou, 510180, China.
³ Department of Otolaryngology, Head and Neck Surgery, The Second Xiangya Hospital, Central South University, Changsha, 410011, China.
⁴ Department of Oncology, Nanfang Hospital, Southern Medical University Guangzhou, 510515, China.
⁵ Department of Cardiology, Nanfang Hospital, Southern Medical University, Guangzhou, 510515, China.
⁶ Department of Oncology, Nanfang Hospital, Southern Medical University Guangzhou, 510515, China. Electronic address: [email protected].

PMID: 28315334
DOI: 10.1016/j.bbrc.2017.03.029

Abstract

The homeoprotein Bapx1 is an important regulator of gastroduodenal tract morphogenesis. Here, we investigated how Bapx1 influences gastric cancer (GC) prognosis and elucidated the underlying mechanisms. Bapx1 expression was greater in GC tissues compared to adjacent non-tumor tissues and expression was positively correlated with mortality, lymph node and distance metastasis. Silencing Bapx1 diminished cell invasion/migration and decreased mesenchymal phenotypes. Transforming growth factor-β (TGF-β) induced Bapx1 expression and epithelial-mesenchymal transition (EMT) in GC cells. However, down-regulated Bapx1 reversed TGF-β induced invasion, migration, morphological changes, and EMT. In summary, Bapx1 indicates poor prognosis for GC by promoting tumor migration and invasion via TGF-β-induced EMT.

Keywords: Bapx1; Epithelial-mesenchymal transition; Gastric cancer; TGF-β.

MeSH terms

Aged
Antigens, CD
Cadherins / genetics
Cadherins / metabolism
Carcinoma / diagnosis
Carcinoma / genetics*
Carcinoma / mortality
Carcinoma / surgery
Cell Line
Cell Line, Tumor
Cell Movement / drug effects
Epithelial-Mesenchymal Transition / genetics*
Female
Gastric Mucosa / drug effects
Gastric Mucosa / metabolism
Gastric Mucosa / pathology
Gene Expression Regulation, Neoplastic*
Homeodomain Proteins / antagonists & inhibitors
Homeodomain Proteins / genetics*
Homeodomain Proteins / metabolism
Humans
Lymphatic Metastasis
Male
Matrix Metalloproteinase 9 / genetics
Matrix Metalloproteinase 9 / metabolism
Middle Aged
Neoplasm Staging
Prognosis
RNA, Small Interfering / genetics
RNA, Small Interfering / metabolism
Signal Transduction
Stomach Neoplasms / diagnosis
Stomach Neoplasms / genetics*
Stomach Neoplasms / mortality
Stomach Neoplasms / surgery
Survival Analysis
Transcription Factors / antagonists & inhibitors
Transcription Factors / genetics*
Transcription Factors / metabolism
Transforming Growth Factor beta / metabolism
Transforming Growth Factor beta / pharmacology*
Vimentin / genetics
Vimentin / metabolism
beta Catenin / genetics
beta Catenin / metabolism

Substances

Antigens, CD
CDH1 protein, human
CTNNB1 protein, human
Cadherins
Homeodomain Proteins
NKX3-2 protein, human
RNA, Small Interfering
Transcription Factors
Transforming Growth Factor beta
Vimentin
beta Catenin
MMP9 protein, human
Matrix Metalloproteinase 9