Abstract
Using an anti-yeast regulatory subunit antibody and the synthetic peptide Kemptide as specific substrate we show in this work that purified preparations of yeast plasma membrane have an associated form of the regulatory subunit and cAMP-dependent protein kinase activity. Treatment of the plasma membrane "in vitro" with 1 microM cAMP releases cAMP-independent protein kinase activity while regulatory subunit remains on the membrane as revealed by immunoblotting. Incubation of the plasma membrane with [gamma-32P]ATP results in the phosphorylation of the regulatory subunit.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Carrier Proteins / metabolism*
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Cell Membrane / enzymology
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Cross Reactions
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Cyclic AMP / pharmacology
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Electrophoresis, Polyacrylamide Gel
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Immunoassay
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Intracellular Signaling Peptides and Proteins*
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Membrane Proteins / analysis
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Oligopeptides / metabolism
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Phosphorylation
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Protein Kinases / metabolism*
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Saccharomyces cerevisiae / enzymology*
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Saccharomyces cerevisiae / ultrastructure
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Substrate Specificity
Substances
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Carrier Proteins
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Intracellular Signaling Peptides and Proteins
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Membrane Proteins
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Oligopeptides
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protein kinase modulator
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kemptide
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Cyclic AMP
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Protein Kinases