Hairy root induction and phytoremediation of textile dye, Reactive green 19A-HE4BD, in a halophyte , Sesuvium portulacastrum (L.) L

Vinayak H Lokhande; Subhash Kudale; Ganesh Nikalje; Neetin Desai; Penna Suprasanna

doi:10.1016/j.btre.2015.08.002

Hairy root induction and phytoremediation of textile dye, Reactive green 19A-HE4BD, in a halophyte , Sesuvium portulacastrum (L.) L

Biotechnol Rep (Amst). 2015 Aug 28:8:56-63. doi: 10.1016/j.btre.2015.08.002. eCollection 2015 Dec.

Authors

Vinayak H Lokhande¹, Subhash Kudale², Ganesh Nikalje³, Neetin Desai⁴, Penna Suprasanna⁵

Affiliations

¹ Shri Shiv Chhatrapati College of Arts, Commerce and Science, Bodkenagar, Junnar, Pune 410 502, India.
² School of Biotechnology and Bioinformatics, Dr. D. Y. Patil University, C. B. D. Belapur, Navi Mumbai 400 614, India.
³ Plant Stress Physiology and Biotechnology Section, Nuclear Agriculture and Biotechnology Division, Bhabha Atomic Research Centre, Trombay, Mumbai 400 085, India; Department of Botany, Savitribai Phule Pune University, Pune 411 007, India.
⁴ School of Biotechnology, Amity University, Mumbai 410 210, India.
⁵ Plant Stress Physiology and Biotechnology Section, Nuclear Agriculture and Biotechnology Division, Bhabha Atomic Research Centre, Trombay, Mumbai 400 085, India.

Abstract

In this study, we report phytoremediation of textile dyes using hairy roots derived through Agrobacterium rhizogenes (NCIM 5140) infection of in vitro leaf and stem explants of a halophyte Sesuvium portulacastrum (L.) L. Leaf explants showed higher frequency of hairy root induction (70%) than stem explants (30%), and maximum number of roots (leaf 42.3 ± 2.4 and stem 50.3 ± 1.7). Transformed nature of hairy roots was ascertained by amplifying 970 bp region of T-DNA of Ri plasmid. Hairy roots were screened for phytoremediation of various textile dyes and results showed that HRs were able to degrade Reactive green 19A HE4BD upto 98% within 5 days of incubation. Spectrophotometric analysis showed decrease in dye concentration while HPLC and FTIR analysis confirmed its degradation. Seed germination assay demonstrated non-toxic nature of the extracted metabolites. This is the first report on induction of hairy root culture in Sesuvium portulacastrum and phytoremediation of textile dyes.

Keywords: ABTS, 2,20-azinobis,3-ethylben-zothiazoline-6-sulfonic acid; Agrobacterium rhizogenes; BA, benzyl adenine; FTIR, Fourier transform infrared spectroscopy; HRs, hairy roots; Hairy root induction; MS, Murashige and Skoog; PCR, polymerase chain reaction; Phytoremediation; Sesuvium portulacastrum (L.) L.; Textile dye; UV–vis, ultra violet visible.