Nutrients and COD removal of swine wastewater with an isolated microalgal strain Neochloris aquatica CL-M1 accumulating high carbohydrate content used for biobutanol production

Yue Wang; Shih-Hsin Ho; Chieh-Lun Cheng; Dillirani Nagarajan; Wan-Qian Guo; Chiayi Lin; Shuangfei Li; Nanqi Ren; Jo-Shu Chang

doi:10.1016/j.biortech.2017.03.122

Nutrients and COD removal of swine wastewater with an isolated microalgal strain Neochloris aquatica CL-M1 accumulating high carbohydrate content used for biobutanol production

Bioresour Technol. 2017 Oct:242:7-14. doi: 10.1016/j.biortech.2017.03.122. Epub 2017 Mar 24.

Authors

Yue Wang¹, Shih-Hsin Ho², Chieh-Lun Cheng³, Dillirani Nagarajan³, Wan-Qian Guo², Chiayi Lin³, Shuangfei Li⁴, Nanqi Ren², Jo-Shu Chang⁵

Affiliations

¹ Shenzhen Key Laboratory of Marine Bioresources and Ecology, Shenzhen University, Shenzhen 518060, China; Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province, College of Optoelectronic Engineering, Shenzhen University, Shenzhen 518060, China.
² State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology, Harbin, China.
³ Department of Chemical Engineering, National Cheng Kung University, Tainan, Taiwan.
⁴ Shenzhen Key Laboratory of Marine Bioresources and Ecology, Shenzhen University, Shenzhen 518060, China.
⁵ State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology, Harbin, China; Department of Chemical Engineering, National Cheng Kung University, Tainan, Taiwan; Research Center for Energy Technology and Strategy, National Cheng Kung University, Tainan 701, Taiwan. Electronic address: [email protected].

PMID: 28377203
DOI: 10.1016/j.biortech.2017.03.122

Abstract

In this study, a carbohydrate-rich microalga Neochloris aquatica CL-M1 was adapted to grow in swine wastewater. The effects of cultivation conditions (i.e., temperature, light intensity or N/P ratio) on COD/nutrients removal and carbohydrate-rich biomass production were investigated. The results indicate that the highest COD removal (81.7%) and NH₃-N removal (96.2%) was achieved at 150µmolm^-2s^-1 light intensity, 25°C and N/P ratio=1.5/1. The highest biomass concentration and carbohydrate content was 6.10gL^-1 and 50.46%, respectively, when N/P ratio=5/1. The resulting carbohydrate-rich microalgal biomass was pretreated and used as a feedstock for butanol fermentation. With the initial sugar concentration of 48.7gL^-1 glucose and 3.4gL^-1 xylose in the pretreated biomass, the butanol concentration, yield, and productivity were 12.0gL^-1, 0.60molmol^-1 sugar, and 0.89gL^-1h^-1, respectively, indicating the high potential of using Neochloris aquatica CL-M1 for butanol fermentation.

Keywords: Butanol fermentation; COD/nutrient removal; Carbohydrate; Microalgae; Neochloris aquatica CL-M1; Swine wastewater.

MeSH terms

Animals
Biomass
Carbohydrates*
Chlorophyta
Microalgae*
Swine
Wastewater*

Substances

Carbohydrates
Waste Water