A novel mitochondria-targeted near-infrared fluorescence probe for ultrafast and ratiometric detection of SO2 derivatives in live cells

Talanta. 2017 Jun 1:168:203-209. doi: 10.1016/j.talanta.2017.03.043. Epub 2017 Mar 18.

Abstract

A novel mitochondria-targeted ratiometric near-infrared fluorescence probe NDMBT for Sulfur dioxide (SO2) derivatives was constructed based on the SO2 derivatives-triggered Michael addition reaction. It displayed ultrafast response time (within 10s), large hypsochromic shift (260nm), high photostability, excellent selectivity and high sensitivity in aqueous media with a detection limit of 43nM. More importantly, it was successfully applied to imaging of the enzymatically generated SO2 derivatives in mitochondria of live cells.

Keywords: Mitochondria-targeted; Near-infrared fluorescence probe; Ratiometric fluorescence detection; SO(2) derivatives.

MeSH terms

  • Cell Proliferation
  • Fluorescence*
  • Fluorescent Dyes / chemistry*
  • HeLa Cells
  • Hep G2 Cells
  • Humans
  • Limit of Detection
  • Mitochondria / metabolism*
  • Molecular Imaging / methods*
  • Sulfur Dioxide / analysis*

Substances

  • Fluorescent Dyes
  • Sulfur Dioxide