Derivation of human iPS cell lines from monozygotic twins in defined and xeno free conditions

Stem Cell Res. 2017 Jan:18:22-25. doi: 10.1016/j.scr.2016.12.006. Epub 2016 Dec 9.

Abstract

Human induced pluripotent stem (hiPS) cell lines CTRL-9-II and CTRL-10-I were derived from healthy monozygotic twin donors using non-integrating RNA based Sendai virus reprogramming and cultured in a xeno-free chemically defined condition. The established hiPS cell lines, CTRL-9-II and CTRL-10-I, are karyotypically normal, free from reprogramming vectors, display endogenously expression of pluripotency factors at levels similar to embryonic stem cells. The generated iPS cell lines demonstrate pluripotency by passing bioinformatics assay PluriTest and by embryonic body assay.

MeSH terms

  • Cell Line
  • Cellular Reprogramming*
  • Culture Media / chemistry*
  • Fibroblasts / cytology
  • Fibroblasts / metabolism
  • Genetic Vectors / genetics
  • Genetic Vectors / metabolism
  • Humans
  • Induced Pluripotent Stem Cells / cytology*
  • Induced Pluripotent Stem Cells / metabolism
  • Karyotype
  • Male
  • RNA, Messenger / metabolism
  • Sendai virus / genetics
  • Transcription Factors / genetics
  • Transcription Factors / metabolism
  • Twins, Monozygotic

Substances

  • Culture Media
  • RNA, Messenger
  • Transcription Factors