Generation of non-integrated induced pluripotent stem cells from a 59-year-old female with multiple endocrine neoplasia type 1 syndrome

Dongsheng Guo; Feima Wu; Haikun Liu; Ge Gao; Shanglong Kou; Fan Yang; Nasir Abbas; Tiancheng Zhou; Xiujuan Cai; Hui Zhang; Dajiang Qin; Jialiang Li; Kecheng Xu; Yin-Xiong Li

doi:10.1016/j.scr.2016.12.009

Generation of non-integrated induced pluripotent stem cells from a 59-year-old female with multiple endocrine neoplasia type 1 syndrome

Stem Cell Res. 2017 Jan:18:64-66. doi: 10.1016/j.scr.2016.12.009. Epub 2016 Dec 9.

Authors

Dongsheng Guo¹, Feima Wu¹, Haikun Liu¹, Ge Gao¹, Shanglong Kou¹, Fan Yang¹, Nasir Abbas¹, Tiancheng Zhou², Xiujuan Cai², Hui Zhang², Dajiang Qin², Jialiang Li³, Kecheng Xu⁴, Yin-Xiong Li⁵

Affiliations

¹ Institute of Public Health, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, China; South China Institute for Stem Cell Biology and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, China.
² South China Institute for Stem Cell Biology and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, China.
³ Guangzhou Fuda Cancer Hospital, Guangzhou City, Guangdong Province, China.
⁴ Guangzhou Fuda Cancer Hospital, Guangzhou City, Guangdong Province, China. Electronic address: [email protected].
⁵ Institute of Public Health, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, China; South China Institute for Stem Cell Biology and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, China; Guangdong Provincial Key Laboratory of Biocomputing, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, China. Electronic address: [email protected].

PMID: 28395808
DOI: 10.1016/j.scr.2016.12.009

Abstract

Urine resource cells were collected from a 59-year-old female patient with multiple endocrine neoplasia type 1 syndrome (MEN1) for generating iPS cells with episomal plasmids carrying Oct4, Sox2, Klf4 and miR-302-367. The patient sustained a heterozygous G>T transition mutation on the exon 9 of Men1 gene that was confirmed by sequencing analysis on the obtained iPSC lines. Karyotyping indicated the chromosomes with normal appearances and numbers. Their pluripotency was demonstrated by gene expression, as well as their abilities for differentiating into three germ layers. This cell line provides an ideal model for studying MEN1.

MeSH terms

Base Sequence
Cell Culture Techniques / methods
Cell Differentiation
Cell Line
Cellular Reprogramming*
DNA Mutational Analysis
Embryoid Bodies / metabolism
Embryoid Bodies / pathology
Exons
Female
Heterozygote
Humans
Induced Pluripotent Stem Cells / cytology*
Induced Pluripotent Stem Cells / metabolism
Induced Pluripotent Stem Cells / transplantation
Karyotype
Kruppel-Like Factor 4
Middle Aged
Multiple Endocrine Neoplasia Type 1 / genetics
Multiple Endocrine Neoplasia Type 1 / metabolism
Multiple Endocrine Neoplasia Type 1 / pathology*
Polymorphism, Single Nucleotide
Proto-Oncogene Proteins / genetics*
Transcription Factors / genetics
Transcription Factors / metabolism
Urine / cytology

Substances

KLF4 protein, human
Kruppel-Like Factor 4
MEN1 protein, human
Proto-Oncogene Proteins
Transcription Factors