Generation of a Nrf2 homozygous knockout human embryonic stem cell line using CRISPR/Cas9

Stem Cell Res. 2017 Mar:19:46-48. doi: 10.1016/j.scr.2016.12.027. Epub 2016 Dec 23.

Abstract

Nuclear factor erythroid 2-related factor 2 (NFE2L2 or Nrf2) is a well-known transcription factor that regulates the expression of a large number of anti-oxidant genes in mammalian cells (J.H. Kim et al., 2014). Here, we generated a homozygous Nrf2 knockout human embryonic stem cell (hESC) line, H9Nrf2KO-A13, using the CRISPR/Cas9 genome editing method. The Nrf2 homozygous knockout H9 cell line maintains pluripotency, differentiation potential into three germ layers, and a normal karyotype.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Base Sequence
  • CRISPR-Cas Systems / genetics*
  • Cell Differentiation
  • Cell Line
  • Female
  • Gene Knockout Techniques
  • Germ Layers / cytology
  • Germ Layers / metabolism
  • Homozygote
  • Human Embryonic Stem Cells / cytology*
  • Human Embryonic Stem Cells / metabolism
  • Humans
  • Karyotype
  • NF-E2-Related Factor 2 / deficiency
  • NF-E2-Related Factor 2 / genetics*
  • Transcription Factors / genetics
  • Transcription Factors / metabolism

Substances

  • NF-E2-Related Factor 2
  • NFE2L2 protein, human
  • Transcription Factors