A homozygous Keap1-knockout human embryonic stem cell line generated using CRISPR/Cas9 mediates gene targeting

Stem Cell Res. 2017 Mar:19:52-54. doi: 10.1016/j.scr.2016.12.028. Epub 2016 Dec 23.

Abstract

Kelch-like ECH-associated protein 1 (keap1) is a cysteine-rich protein that interacts with transcription factor Nrf2 in a redox-sensitive manner, leading to the degradation of Nrf2 (Kim et al., 2014a). Disruption of Keap1 results in the induction of Nrf2-related signaling pathways involving the expression of a set of anti-oxidant and anti-inflammatory genes. We generated biallelic mutants of the Keap1 gene using a CRISPR-Cas9 genome editing method in the H9 human embryonic stem cell (hESC). The Keap1 homozygous-knockout H9 cell line retained normal morphology, gene expression, and in vivo differentiation potential.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Base Sequence
  • CRISPR-Cas Systems / genetics*
  • Cell Line
  • Gene Knockout Techniques
  • Homozygote
  • Human Embryonic Stem Cells / cytology*
  • Human Embryonic Stem Cells / metabolism
  • Humans
  • Karyotype
  • Kelch-Like ECH-Associated Protein 1 / deficiency
  • Kelch-Like ECH-Associated Protein 1 / genetics*
  • Microscopy, Fluorescence

Substances

  • KEAP1 protein, human
  • Kelch-Like ECH-Associated Protein 1