The various modules in multimodular carbohydrate-active enzymes (CAZymes) may function in catalysis, carbohydrate binding, protein-protein interactions or as linkers. Here, we describe how combining the biophysical techniques of Small Angle X-ray Scattering (SAXS) and macromolecular X-ray crystallography (XRC) provides a powerful tool for examination into questions related to overall structural organization of ultra multimodular CAZymes.
Keywords: CAZymes; CBM; Carbohydrate binding module; Carbohydrate-active enzymes; Cohesin; Dissect and build; Dockerin; Glycoside hydrolase; Multimodular; SAXS; Small-angle X-ray scattering; Structure; X-ray Crystallography.