Flow cytometric analysis of the ERCC1, marker of DNA damage repair, in the tumor specimens embedded into paraffin blocks

Dokl Biochem Biophys. 2017 Jan;472(1):9-11. doi: 10.1134/S1607672917010033. Epub 2017 Apr 19.

Abstract

The differences in expression of ERCC1 were estimated between tumor specimens embedded into paraffin blocks and surgical biopsy specimens of non-small cell lung cancer as well as breast and ovarian cancers. Concordance or differences not higher than 20% were observed in 73% of the cases. The number of the cases with more significant differences in ERCC1 expression was less than 17%. The results show that ERCC1 detection in surgical biopsy specimens by flow cytometry is the more preferable method due to reduced preanalytical phase of the analysis.

Publication types

  • Comparative Study

MeSH terms

  • Biomarkers, Tumor / metabolism*
  • Breast Neoplasms / metabolism*
  • Breast Neoplasms / pathology
  • Carcinoma, Non-Small-Cell Lung / genetics*
  • Carcinoma, Non-Small-Cell Lung / pathology
  • DNA-Binding Proteins / metabolism*
  • Endonucleases / metabolism*
  • Female
  • Flow Cytometry / methods*
  • Flow Cytometry / standards
  • Humans
  • Molecular Diagnostic Techniques / methods*
  • Molecular Diagnostic Techniques / standards
  • Ovarian Neoplasms / metabolism*
  • Ovarian Neoplasms / pathology
  • Paraffin Embedding / methods
  • Paraffin Embedding / standards

Substances

  • Biomarkers, Tumor
  • DNA-Binding Proteins
  • ERCC1 protein, human
  • Endonucleases