Appendix G: Vitrification of Blastocysts Using VitriBlastTM and ThermoBlastTM: Nidacon

Anna Niläng Laessker; Thorir Hardarsson; Ann-Sofie Forsberg; Tetsunori Mukaida; Paul V Holmes

doi:10.1007/978-1-4939-6828-2_27

Appendix G: Vitrification of Blastocysts Using VitriBlast^TM and ThermoBlast^TM: Nidacon

Methods Mol Biol. 2017:1568:355-365. doi: 10.1007/978-1-4939-6828-2_27.

Authors

Anna Niläng Laessker¹, Thorir Hardarsson², Ann-Sofie Forsberg³, Tetsunori Mukaida⁴, Paul V Holmes³

Affiliations

¹ Product Development, Nidacon International AB, Flöjelbergsgatan 16b, 43137, Mölndal, Sweden. [email protected].
² Fertility Center, Carlanderska Hospital, 402 29, Gothenburg, Sweden.
³ Product Development, Nidacon International AB, Flöjelbergsgatan 16b, 43137, Mölndal, Sweden.
⁴ Hiroshima Hart Clinic, 5-7-10 Ohtemachi Nakaku, Hiroshima, 7300051, Japan.

PMID: 28421511
DOI: 10.1007/978-1-4939-6828-2_27

Abstract

This appendix describes the vitrification of blastocysts using VitriBlast™ (VBK) and ThermoBlast™ (TBK) from Nidacon, Mölndal, Sweden. The technique used and the reason for not including DMSO in the medium at the production stage, but including it separately in the kit, and the importance of collapsing the blastocyst prior to vitrification will be explained and described.

Keywords: Blastocysts; Collapsing; Cryopreservation; DMSO; Ethylene glycol; Nidacon; ThermoBlast™; VitriBlast™; Vitrification.

MeSH terms

Animals
Blastocyst* / cytology
Cryopreservation / methods*
Cryoprotective Agents*
Female
Humans
Vitrification*

Substances

Cryoprotective Agents