Expression of varicella-zoster virus glycoprotein I in cells infected with a vaccinia virus recombinant

Virus Res. 1988 May;10(2-3):205-13. doi: 10.1016/0168-1702(88)90016-0.

Abstract

BSC-1 cells infected with a vaccinia virus recombinant containing the coding sequences for varicella-zoster virus (VZV) glycoprotein I (gpI) were analyzed by indirect immunofluorescence and immunoprecipitation for the expression and processing of gpI. The processing of gpI in cells infected with recombinant virus was the same as that observed during VZV infection. Immunofluorescence revealed localization of gpI to the membranes of recombinant virus-infected cells.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Cell Line
  • Cloning, Molecular
  • Electrophoresis, Polyacrylamide Gel
  • Fluorescent Antibody Technique
  • Gene Expression Regulation*
  • Genetic Vectors
  • Herpesvirus 3, Human / genetics*
  • Immunoassay
  • Vaccinia virus / genetics*
  • Viral Envelope Proteins*
  • Viral Proteins / biosynthesis
  • Viral Proteins / genetics*

Substances

  • Viral Envelope Proteins
  • Viral Proteins
  • glycoprotein E, varicella-zoster virus