Pharmacogenetic analysis of hepatitis C virus related mixed cryoglobulinemia

Pharmacogenomics. 2017 May;18(7):607-611. doi: 10.2217/pgs-2016-0040. Epub 2017 Apr 28.

Abstract

Aim: Mixed cryoglobulinemia (MC) is an extra hepatic hepatitis C virus related problem and different studies suggested genetics' role in predicting this complication. We evaluated the influence of SNPs in IL-28B, SLC29A1, SLC28A2, NT5C2, HNF4 and ABCB1 genes in MC prediction.

Patients & methods: SNPs were evaluated through real-time PCR.

Results: ABCB1 (gene encoding P-glycoprotein) 3435C>T SNP was associated with MC presence (p = 0.034): related to T allele carriers (CC vs CT/TT), we reached a p-value of 0.013. In the logistic regression analysis baseline viral load >600.000 IU/ml (p < 001), IL28B rs8099917/rs12979860 TT/CC (p < 0.001), NT5 (gene encoding for 5' nucleotidase) 153 TC (p = 0.012) and ABCB1 3435 CT/TT (p = 0.034) genotypes predicted MC presence.

Conclusion: These data could help clinicians to identify patients with higher probability to show MC extra hepatic complication.

Keywords: ABCB1; HCV; HCV extra hepatic manifestation; NT5; SNPs; cryoglobulin; pegylated interferon/ribavirin; pharmacogenetics.

MeSH terms

  • Adult
  • Cryoglobulinemia / diagnosis
  • Cryoglobulinemia / epidemiology*
  • Cryoglobulinemia / genetics*
  • Female
  • Hepacivirus / genetics
  • Hepacivirus / isolation & purification
  • Hepatitis C, Chronic / diagnosis
  • Hepatitis C, Chronic / epidemiology*
  • Hepatitis C, Chronic / genetics*
  • Humans
  • Male
  • Middle Aged
  • Pharmacogenomic Testing / methods*
  • Polymorphism, Single Nucleotide / genetics*
  • Retrospective Studies