NDM-5 and OXA-181 Beta-Lactamases, a Significant Threat Continues To Spread in the Americas

Laura J Rojas; Andrea M Hujer; Susan D Rudin; Meredith S Wright; T Nicholas Domitrovic; Steven H Marshall; Kristine M Hujer; Sandra S Richter; Eric Cober; Federico Perez; Mark D Adams; David van Duin; Robert A Bonomo

doi:10.1128/AAC.00454-17

NDM-5 and OXA-181 Beta-Lactamases, a Significant Threat Continues To Spread in the Americas

Antimicrob Agents Chemother. 2017 Jun 27;61(7):e00454-17. doi: 10.1128/AAC.00454-17. Print 2017 Jul.

Authors

Laura J Rojas^{1

2}, Andrea M Hujer^{1

3}, Susan D Rudin^{1

3}, Meredith S Wright⁴, T Nicholas Domitrovic^{1

3}, Steven H Marshall¹, Kristine M Hujer^{1

3}, Sandra S Richter⁵, Eric Cober⁶, Federico Perez^{7

3}, Mark D Adams⁴, David van Duin⁸, Robert A Bonomo^{9

1

3

2

10

11

12}

Affiliations

¹ Research Service, Louis Stokes Cleveland Department of Veterans Affairs Medical Center, Cleveland, Ohio, USA.
² Department of Molecular Biology and Microbiology, Case Western Reserve University School of Medicine, Cleveland, Ohio, USA.
³ Department of Medicine, Case Western Reserve University School of Medicine, Cleveland, Ohio, USA.
⁴ J. Craig Venter Institute, La Jolla, California, USA.
⁵ Department of Laboratory Medicine, Cleveland Clinic, Cleveland, Ohio, USA.
⁶ Department of Infectious Diseases, Cleveland Clinic, Cleveland, Ohio, USA.
⁷ Medical Service, Louis Stokes Cleveland Department of Veterans Affairs Medical Center, Cleveland, Ohio, USA.
⁸ Division of Infectious Diseases, University of North Carolina, Chapel Hill, North Carolina, USA [email protected] [email protected].
⁹ Medical Service, Louis Stokes Cleveland Department of Veterans Affairs Medical Center, Cleveland, Ohio, USA [email protected] [email protected].
¹⁰ Department of Biochemistry, Case Western Reserve University School of Medicine, Cleveland, Ohio, USA.
¹¹ Department of Pharmacology, Case Western Reserve University School of Medicine, Cleveland, Ohio, USA.
¹² Department of Proteomics and Bioinformatics, Case Western Reserve University School of Medicine, Cleveland, Ohio, USA.

Abstract

Among Gram-negative bacteria, carbapenem-resistant infections pose a serious and life-threatening challenge. Here, the CRACKLE network reports a sentinel detection and characterization of a carbapenem-resistant Klebsiella pneumoniae ST147 isolate harboring bla_NDM-5 and bla_OXA-181 from a young man who underwent abdominal surgery in India. bla_NDM-5 was located on an IncFII plasmid of ≈90 kb, whereas bla_OXA-181 was chromosomally encoded. Resistome and genome analysis demonstrated multiple copies of the transposable element IS26 and a "hot-spot region" in the IncFII plasmid.

Keywords: Klebsiella pneumoniae; NDM-5; OXA-181.

MeSH terms

Anti-Bacterial Agents / pharmacology
Anti-Bacterial Agents / therapeutic use
Carbapenems / pharmacology
Carbapenems / therapeutic use
Humans
India
Klebsiella Infections / drug therapy
Klebsiella Infections / microbiology
Klebsiella pneumoniae / enzymology*
Klebsiella pneumoniae / pathogenicity*
Male
Microbial Sensitivity Tests
Plasmids / genetics
beta-Lactamases / genetics
beta-Lactamases / metabolism*

Substances

Anti-Bacterial Agents
Carbapenems
beta-Lactamases

Abstract

MeSH terms

Substances

Grants and funding