Abstract
A bidirectional expression vector containing both the 11KD late promoter (p11) and the presumptive 25KD early promoter (p25) was constructed. These bidirectional vectors have been applied to the expression of hepatitis B surface antigen by using one of the promoters for beta-galactosidase as the marker gene and the other one for hepatitis B surface antigen as the target gene.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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DNA Restriction Enzymes
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Gene Expression Regulation*
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Genetic Markers
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Genetic Vectors*
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Hepatitis B Surface Antigens / genetics
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Plasmids*
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Promoter Regions, Genetic*
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Vaccinia virus / genetics*
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beta-Galactosidase / genetics
Substances
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Genetic Markers
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Hepatitis B Surface Antigens
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DNA Restriction Enzymes
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beta-Galactosidase