Nonenzymatic Oligomerization of 3',5'-Cyclic CMP Induced by Proton and UV Irradiation Hints at a Nonfastidious Origin of RNA

Chembiochem. 2017 Aug 4;18(15):1535-1543. doi: 10.1002/cbic.201700122. Epub 2017 Jun 30.

Abstract

We report that 3',5'-cyclic CMP undergoes nonenzymatic di- and trimerization at 20 °C under dry conditions upon proton or UV irradiation. The reaction involves stacking of the cyclic monomers and subsequent polymerization through serial transphosphorylations between the stacked monomers. Proton- and UV-induced oligomerization of 3',5'-cyclic CMP demonstrates that pyrimidines-similar to purines-might also have taken part in the spontaneous generation of RNA under plausible prebiotic conditions as well as in an extraterrestrial context. The observed polymerization of naturally occurring 3',5'-cyclic nucleotides supports the possibility that the extant genetic nucleic acids might have originated by way of a straight Occamian path, starting from simple reactions between plausibly preactivated monomers.

Keywords: RNA; cyclic CMPs; nucleotides; polymerization; proton and UV irradiation.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Circular Dichroism
  • Cyclic CMP / chemistry*
  • Cyclic CMP / radiation effects*
  • Evolution, Chemical
  • Models, Chemical
  • Oligoribonucleotides / chemical synthesis*
  • Polymerization
  • Protons
  • RNA / chemical synthesis*
  • Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
  • Ultraviolet Rays

Substances

  • Oligoribonucleotides
  • Protons
  • Cyclic CMP
  • RNA