Sensitivity of the LuminoTox tool to monitor contaminants of emerging concern in municipal secondary wastewater effluent

Contaminants of emerging concern (CECs) are generally poorly removed during conventional wastewater treatment. There is a need for rapid, sensitive and inexpensive methods to monitor the quality of treated wastewater effluent. The purpose of this study was to assess the applicability of the LuminoTox as a tool to monitor municipal secondary effluent (SE) and to determine its sensitivity to the presence of CECs. The effect of exposure method on a 14 CECs mix was explored; 20min in the dark or 30min under light were both recommended as they were sensitive to the detection of CECs in wastewater while providing a short run time. Stabilized aqueous photosynthetic systems (SAPS) detected the 14 CECs mix in the wastewater matrices when they were present at a concentration in the 6μg/L to 50μg/L range. Interference on the biosensors were examined in a range of wastewater characteristics commonly observed in SE and, for most cases, biosensors were not inhibited which suggests that, in most cases, wastewater characteristics would not cause toxic interferences. SAPS detected CECs in SE with different modes of action with the degree of sensitivity of individual CECs developed from experimental and literature values as follows: inhibitors of the plastoquinone binding site within photosystem II>direct or indirect inhibitors of photosynthesis acting on binding sites other than that of the Q_B. SAPS were assessed for their ability to detect residual CECs in SE without sample preparation, however, the effluent examined exhibited minimal inhibition for SAPS II (7±1%) and no inhibition for SAPS I. These results highlight the need for the development of a sample pre-concentration method to increase the biosensor sensitivity towards native CECs. This would allow the LuminoTox to be an effective tool for monitoring wastewater quality with the intent of residual CECs detection.