Genomic and Epidemiological Evidence for Community Origins of Hospital-Onset Methicillin-Resistant Staphylococcus aureus Bloodstream Infections

J Infect Dis. 2017 Jun 1;215(11):1640-1647. doi: 10.1093/infdis/jiw647.

Abstract

Background: We examined whether disparities existed in hospital-onset (HO) Staphylococcus aureus bloodstream infections (BSIs) and used whole-genome sequencing (WGS) to identify factors associated with USA300 transmission networks.

Methods: We evaluated HO methicillin-susceptible S. aureus (MSSA) and HO methicillin-resistant S. aureus (MRSA) BSIs for 2009-2013 at 2 hospitals and used an adjusted incidence for modeling. WGS and phylogenetic analyses were performed on a sample of USA300 BSI isolates. Epidemiologic data were analyzed in the context of phylogenetic reconstructions.

Results: On multivariate analysis, male sex, African-American race, and non-Hispanic white race/ethnicity were significantly associated with HO-MRSA BSIs whereas Hispanic ethnicity was negatively associated (rate ratio, 0.41; P = .002). Intermixing of community-onset and HO-USA300 strains on the phylogenetic tree indicates that these strains derive from a common pool. African-American race was the only factor associated with genomic clustering of isolates.

Conclusions: In a multicenter assessment of HO-S. aureus BSIs, African-American race was significantly associated with HO-MRSA but not MSSA BSIs. There appears to be a nexus of USA300 community and hospital transmission networks, with a community factor being the primary driver. Our data suggest that HO-USA300 BSIs likely are due to colonizing strains acquired in the community before hospitalization. Therefore, prevention efforts may need to extend to the community for maximal benefit.

Keywords: MRSA; Whole genome sequencing; bacteremia; disparities.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Bacteremia* / epidemiology
  • Bacteremia* / microbiology
  • Bacteremia* / transmission
  • Cross Infection* / epidemiology
  • Cross Infection* / microbiology
  • Cross Infection* / transmission
  • DNA, Bacterial / analysis
  • DNA, Bacterial / genetics
  • Female
  • Genome, Bacterial / genetics
  • Genomics
  • Humans
  • Male
  • Methicillin-Resistant Staphylococcus aureus / genetics*
  • Retrospective Studies
  • Sequence Analysis, DNA
  • Staphylococcal Infections* / epidemiology
  • Staphylococcal Infections* / microbiology
  • Staphylococcal Infections* / transmission

Substances

  • DNA, Bacterial