We report here the successful 3D visualization of human placenta villous structures on the order of ∼1 mm3 by a combination of immunolabeling, rapid tissue clarification and laser scanning confocal microscopy. The resultant image sets exhibit a complex arrangement of villi and their contained vasculature that mirrors their arrangement in situ.
Keywords: Capillary network; Confocal microscopy; Immunofluorescence; Placenta; Tissue clarification.
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