Identification and characterization of glucagon-like peptide-1 7-36 amide-binding sites in the rat brain and lung

FEBS Lett. 1988 Dec 5;241(1-2):209-12. doi: 10.1016/0014-5793(88)81063-9.

Abstract

High-affinity binding sites for glucagon-like peptide-1 7-36 amide (GLP-1 7-36 NH2) were identified in rat brain and lung membranes. Binding of [125I]GLP-1 7-36 NH2 was rapid, reversible, specific, saturable and pH dependent. Specific binding in the central nervous system was particularly high in the hypothalamus and the brain stem. Oxyntomodulin, glucagon-like peptide-1, glucagon-like peptide-2 and glucagon were 100-1000-fold less potent than GLP-1 7-36 NH2 in competition for this binding site.

MeSH terms

  • Animals
  • Binding, Competitive
  • Brain / metabolism*
  • Glucagon / metabolism*
  • Glucagon-Like Peptide 1
  • Glucagon-Like Peptides
  • Kinetics
  • Lung / metabolism*
  • Organ Specificity
  • Peptide Fragments*
  • Peptides / metabolism*
  • Rats
  • Rats, Inbred Strains
  • Receptors, Gastrointestinal Hormone / metabolism*
  • Receptors, Glucagon

Substances

  • Peptide Fragments
  • Peptides
  • Receptors, Gastrointestinal Hormone
  • Receptors, Glucagon
  • glucagon-like peptide 1 (7-36)amide
  • Glucagon-Like Peptides
  • Glucagon-Like Peptide 1
  • Glucagon