Preheating of urine improves the specificity of urinary cryptococcal antigen testing using the lateral flow assay

Fábio Brito-Santos; Marcela de Faria Ferreira; Luciana Trilles; Mauro de Medeiros Muniz; Valdiléa Gonçalves Veloso Dos Santos; Filipe Anibal Carvalho-Costa; Wieland Meyer; Bodo Wanke; Márcia Dos Santos Lazéra

doi:10.1371/journal.pntd.0005304

Preheating of urine improves the specificity of urinary cryptococcal antigen testing using the lateral flow assay

PLoS Negl Trop Dis. 2017 May 11;11(5):e0005304. doi: 10.1371/journal.pntd.0005304. eCollection 2017 May.

Authors

Fábio Brito-Santos¹, Marcela de Faria Ferreira¹, Luciana Trilles¹, Mauro de Medeiros Muniz¹, Valdiléa Gonçalves Veloso Dos Santos¹, Filipe Anibal Carvalho-Costa², Wieland Meyer^{1

3}, Bodo Wanke¹, Márcia Dos Santos Lazéra¹

Affiliations

¹ National Institute of Infectious Diseases Evandro Chagas (INI), FIOCRUZ, Rio de Janeiro, Brazil.
² Oswaldo Cruz Institute, FIOCRUZ, Rio de Janeiro, Brazil.
³ Centre for Infectious Diseases and Microbiology, Sydney Medical School-Westmead Hospital, Marie Bashir Institute for Infectious Diseases and Biosecurity, The University of Sydney, Westmead Institute for Medical Research, Sydney, New South Wales, Australia.

No abstract available

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

AIDS-Related Opportunistic Infections / diagnosis
AIDS-Related Opportunistic Infections / urine
Antigens, Fungal / immunology
Antigens, Fungal / urine*
Cryptococcosis / complications
Cryptococcosis / diagnosis*
Cryptococcosis / urine*
Cryptococcus neoformans / metabolism*
HIV Infections / complications
Hot Temperature*
Humans
Point-of-Care Systems
Sensitivity and Specificity
Urinalysis / methods*

Substances

Antigens, Fungal

Grants and funding

The study was partially supported by the “Coordenação de Aperfeiçoamento de Pessoal de Nivel Superior” (CAPES) grant # 098/2012 to BW, CNPq-PAPES/Fiocruz grant # 407565/2012 4 to MdSL, the program science without borders (CAPES), Brazil, grant # AUXPE-PVE 0251/2013 to WM. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.