Does copy number variation of APOL1 gene affect the susceptibility to focal segmental glomerulosclerosis?

Ren Fail. 2017 Nov;39(1):500-504. doi: 10.1080/0886022X.2017.1323646.

Abstract

Background: APOL1 risk variants (G1 and G2) are associated with increased susceptibility to focal segmental glomerulosclerosis (FSGS) in African population. However, the two risk mutations were not found in Chinese FSGS patients. In this study, we explored the association between the copy number variation (CNV) of APOL1 gene and FSGS.

Methods: APOL1 copy number variations were detected by quantitative real-time PCR with TaqMan probes and compared between 133 FSGS patients and 123 controls. The association between CNV of APOL1 gene and clinical parameters was also investigated.

Results: The distribution of APOL1 CNV did not show significant difference between FSGS patients and controls. The creatinine and proteinuria in the high copy number group (CN ≥ 3) were higher than the other two groups, but the difference was not significant (p > .05). The FSGS pathological types were different among the three groups.

Conclusion: There was no significant difference in the distribution of APOL1 gene copy variants between FSGS patients and normal controls, and there was no significant correlation between the APOL1 gene CNV and the FSGS patients' clinical manifestations. APOL1 CNVs may be not associated with susceptibility to FSGS.

Keywords: APOL1; Focal segmental glomerulosclerosis; copy number variation.

MeSH terms

  • Adolescent
  • Adult
  • Aged
  • Apolipoprotein L1 / genetics*
  • Asian People / genetics
  • Biopsy
  • DNA Copy Number Variations*
  • Female
  • Genetic Predisposition to Disease / genetics*
  • Glomerulosclerosis, Focal Segmental / genetics*
  • Glomerulosclerosis, Focal Segmental / pathology
  • Humans
  • Kidney / pathology
  • Male
  • Middle Aged
  • Real-Time Polymerase Chain Reaction
  • Risk Factors
  • Young Adult

Substances

  • APOL1 protein, human
  • Apolipoprotein L1

Grants and funding

This study was supported in part by National Basic Research Program of China 973 (No. 2012CB517600, 2012CB517604), Youth Science and Technology Creative Research Groups of Sichuan Province (2015TD0013) and National Natural Science Foundation of China (No. 81300618).