A Color Segmentation-Based Method to Quantify Atherosclerotic Lesion Compositions with Immunostaining

Methods Mol Biol. 2017:1614:21-30. doi: 10.1007/978-1-4939-7030-8_2.

Abstract

There is an increasing recognition that atherosclerotic lesion composition, rather than size, is the determinant of acute events. Immunostaining is a commonly used method to characterize atherosclerotic lesion compositions. Here, we describe a color segmentation-based approach in HSI (hue, saturation, and intensity) color mode, which minimizes subjectivity and produces accurate and consistent quantifications of atherosclerotic lesion compositions.

Keywords: Antibody; Atherosclerosis; Color; Imaging; Immunostaining.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, N.I.H., Extramural

MeSH terms

  • Animals
  • Atherosclerosis / diagnosis*
  • Atherosclerosis / metabolism
  • Biomarkers / metabolism
  • Disease Models, Animal*
  • Image Interpretation, Computer-Assisted / methods*
  • Immunoenzyme Techniques / methods*
  • Male
  • Mice
  • Mice, Knockout
  • Pattern Recognition, Automated / methods*
  • Receptors, LDL / physiology
  • Staining and Labeling / methods*

Substances

  • Biomarkers
  • Receptors, LDL