CCR2 Regulates the Immune Response by Modulating the Interconversion and Function of Effector and Regulatory T Cells

J Immunol. 2017 Jun 15;198(12):4659-4671. doi: 10.4049/jimmunol.1601458. Epub 2017 May 15.

Abstract

Chemokines and chemokine receptors establish a complex network modulating immune cell migration and localization. These molecules were also suggested to mediate the differentiation of leukocytes; however, their intrinsic, direct regulation of lymphocyte fate remained unclear. CCR2 is the main chemokine receptor inducing macrophage and monocyte recruitment to sites of inflammation, and it is also expressed on T cells. To assess whether CCR2 directly regulates T cell responses, we followed the fates of CCR2-/- T cells in T cell-specific inflammatory models. Our in vitro and in vivo results show that CCR2 intrinsically mediates the expression of inflammatory T cell cytokines, and its absence on T cells results in attenuated colitis progression. Moreover, CCR2 deficiency in T cells promoted a program inducing the accumulation of Foxp3+ regulatory T cells, while decreasing the levels of Th17 cells in vivo, indicating that CCR2 regulates the immune response by modulating the effector/regulatory T ratio.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cell Movement
  • Colitis / immunology
  • Cytokines / genetics
  • Cytokines / immunology
  • Forkhead Transcription Factors / genetics
  • Forkhead Transcription Factors / metabolism
  • Gene Expression Regulation
  • Immunity, Cellular*
  • Macrophages / immunology
  • Mice
  • Receptors, CCR2 / deficiency
  • Receptors, CCR2 / genetics
  • Receptors, CCR2 / immunology
  • Receptors, CCR2 / metabolism*
  • Receptors, CCR5 / immunology
  • Receptors, CCR5 / metabolism
  • T-Lymphocytes, Regulatory / immunology*
  • Th17 Cells / immunology
  • Th17 Cells / physiology

Substances

  • Ccr2 protein, mouse
  • Cytokines
  • Forkhead Transcription Factors
  • Foxp3 protein, mouse
  • Receptors, CCR2
  • Receptors, CCR5