Abstract
The N-terminal fragment, comprising residues -5 to 55 of herpes simplex virus type 1 glycoprotein D was expressed as a beta-galactosidase fusion protein in Escherichia coli. This gD-fusion protein reacts with monoclonal antibody LP 14 directed against glycoprotein D of HSV. Antisera obtained after immunization of rabbits with purified gD-fusion protein react with HSV-1 gD in a Western blot and with N-terminal synthetic peptides of gD. In addition, these antisera are able to neutralize viral infectivity in vitro.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Animals
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Antibodies, Viral / biosynthesis
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Antibodies, Viral / immunology
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Chinchilla
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Cloning, Molecular
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Escherichia coli / genetics
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Molecular Sequence Data
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Plasmids
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Rabbits
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Recombinant Fusion Proteins / biosynthesis
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Recombinant Fusion Proteins / immunology*
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Recombinant Proteins / immunology*
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Simplexvirus / genetics
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Simplexvirus / immunology*
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Viral Envelope Proteins / biosynthesis
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Viral Envelope Proteins / genetics
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Viral Envelope Proteins / immunology*
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beta-Galactosidase / genetics
Substances
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Antibodies, Viral
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Recombinant Fusion Proteins
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Recombinant Proteins
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Viral Envelope Proteins
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glycoprotein D, Human herpesvirus 1
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beta-Galactosidase