Abstract
The expression of 20 known cellular proto-oncogenes in human well-differentiated hepatoma cell line Hep3B and poorly-differentiated hepatoma cell line HA22T/VGH was studied by Northern blot hybridization. Among the cellular proto-oncogenes examined, both cell lines express protein kinase genes including fps, mos and raf; PDGF B chain sis gene; GTP/GDP binding protein gene Ha-ras and nuclear protein genes including fos and myc. The expression of yes, abl, ros, src, erb-B, erb-A, fms, Ki-ras, myb, rel and bas genes was not detected in both cell lines.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Blotting, Northern
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Carcinoma, Hepatocellular / genetics*
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Carcinoma, Hepatocellular / pathology
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Cell Differentiation
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Cell Line
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DNA Probes
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GTP-Binding Proteins / genetics
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Humans
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Liver Neoplasms / genetics*
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Liver Neoplasms / pathology
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Nucleic Acid Hybridization
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Platelet-Derived Growth Factor / genetics
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Protein Kinases / genetics
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Proto-Oncogene Proteins / genetics
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Proto-Oncogene Proteins c-sis
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Proto-Oncogenes*
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RNA, Neoplasm / analysis
Substances
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DNA Probes
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Platelet-Derived Growth Factor
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Proto-Oncogene Proteins
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Proto-Oncogene Proteins c-sis
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RNA, Neoplasm
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Protein Kinases
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GTP-Binding Proteins