Alpha- and Beta-Diastereoisomers of Phenylcobalamin from Cobalt-Arylation with Diphenyliodonium Chloride

Chemistry. 2017 Jul 21;23(41):9726-9731. doi: 10.1002/chem.201701514. Epub 2017 Jun 28.

Abstract

Organometallic aryl-cobalamins are B12 -derivatives featuring properties of potential 'B12 antivitamins'. Herein, we describe a new method for the preparation of aryl-cobalamins using versatile diaryliodonium salts as arylation agents. Formate or sodium borohydride reduction of aquocobalamin in presence of diphenyliodonium chloride furnished Coβ -phenyl-cobalamin PhCbl in a roughly 3:1 to 1:1 ratio with its coordination isomer αPhCbl, a first representative 'base-off' Coα -aryl-cobalamin. The new structures were secured by detailed spectroscopic analysis, supplemented by an X-ray crystal structure analysis of PhCbl. Both types of coordination isomers of the aryl-cobalamins promise to be useful molecular tools in biomedical and biological studies.

Keywords: antivitamin b12; arylation; iodonium salt; radicals; vitamins.

MeSH terms

  • Biphenyl Compounds / chemistry*
  • Circular Dichroism
  • Cobalt / chemistry*
  • Crystallography, X-Ray
  • Molecular Conformation
  • Onium Compounds / chemistry*
  • Spectrophotometry
  • Stereoisomerism
  • Vitamin B 12 / chemical synthesis
  • Vitamin B 12 / chemistry

Substances

  • Biphenyl Compounds
  • Onium Compounds
  • diphenyliodonium
  • Cobalt
  • Vitamin B 12